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- 规格：
200 ul
predicted_size：null
Uniprot ID：Q6UXV
description：GFRAL (C-term) rabbit polyclonal antibody, Aff - Purified
clonality：Polyclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：null
immunogen：KLH conjugated synthetic peptide between 366-394 amino acids from the C-terminal region of human GFRAL
recommended_dilution： ELISA: 1/1000. Western Blot: 1/100-1/500. Flow Cytometry: 1/10-1/50. Immunohistochemistry on Paraffin Sections: 1/10-1/50.
predicted_size：null
buffer：PBS containing 0.09% (W/V) Sodium Azide as preservative; State: Aff - Purified; State: Liquid purified Ig fraction
host_species：Rabbit
applications：FC, IHC, WB
reactivities：Human
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文献和实验Use of EIA to Measure MMPs and TIMPs
the combination of a monoclonal antibody as the solid-phase with rabbit polyclonal antibody and horseradish peroxidase (HRP)-labeled donkey antirabbit gamma globulin as the second phase (2 ). EIAs for human MMP-9 were developed using MMP-9 specific polyclonal
Tips and hints for the storage of antibodies
! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses. Rabbit antisera Antisera are more robust than monoclonals. With anti-microbials added, they may be stored at ℃. Serum does not contain active proteases
Immunocytology using the HA epitope
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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