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- OriGene
- AP33395PU-N
- 2025年07月12日
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- 文献和实验
- 技术资料
- 规格：
100 ug
predicted_size：~ 11 kDa
Uniprot ID：P1148
description：GnRH (GNRH1) (11-60) rabbit polyclonal antibody, Aff - Purified
clonality：Polyclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：GnRH is produced by hypothalamic neurons and secreted in a pulsatile manner into the capillary plexus of the median eminence. GnRH affects the release of lutenizing hormone and follicle stimulating hormone from gonadotropic cells in the anterior pituitary. In addition to hypothalamic GnRH (GnRH I), a second GnRH form (GnRH II) functions primarily in the midbrain. GnRH is xpressed in the acrosomal region of human sperm and in the anterior pituitary tissue and cancer cells. Unlike GnRH I, GnRH II is highly expressed outside the brain, particularly in the kidney, bone marrow and prostate, suggesting that it may have multiple functions. GnRH binds to a specific G protein-coupled receptor in the pituitary to regulate synthesis and secretion of gonadotropins.
immunogen：Synthetic peptide corresponding to amino acids 11-60 of Human GnRH I.
recommended_dilution：Western blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
predicted_size：~ 11 kDa
buffer：PBS, pH 7.2; State: Aff - Purified; State: Liquid purified Ig fraction (> 95% by SDS-PAGE); Preservative: 0.05% Sodium Azide
host_species：Rabbit
applications：IHC, WB
reactivities：Human, Mouse, Rat
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文献和实验Wholemount staining of embryos
supernatent, in which case it is used either neat or diluted with blocking solution, or as ascites or polyclonal in which case it is diluted in blocking solution. The dilution depends on the antibody. Ascites can often be diluted 1/2000 or more. Polyclonals
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
Reagents Ammonium sulfate (50% [w/v]) (optional, see Step 23) Antibodies, primary Affinity-purified primary antibodies are diluted in PBS containing 1% BSA. The dilutions must be adjusted for each individual primary antibody. Typical
protein depends upon the species that the antibody was raised in. Protein A binds well to rabbit, cat, human, pig and guinea pig IgG as well as mouse IgG2a and IgG2b. Protein G binds strongly to IgG from cow, goat, sheep, cow, horse, rabbit and guinea pig
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