GFP chicken polyclonal antibod

gene_symbol：GFP
predicted_size：null
Uniprot ID：P42212
description：GFP chicken polyclonal antibody, Aff - Purified
clonality：Polyclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
immunogen：Purified recombinant Green Fluorescent Protein (GFP) emulsified in Freund's adjuvant. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks. Purified antibody preparations were then mixed with Glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with Thimerosal, and then filter-sterilized.
recommended_dilution：Western Blot (1/2500-1/5000).  Immuofluorescence. Immunohistochemistry (1/500-1/1000).
predicted_size：null
buffer：Sodium Phosphate-Buffered (10 mM, pH7.2) isotonic (0.9%, w/v) Saline with 50% (v/v) Glycerol as an anti-freezing agent and 0.02% (w/v) sodium azide as an anti-microbial agent.; State: Aff - Purified; State: Liquid purified IgY fraction
host_species：Chicken
applications：IF, IHC, WB
reactivities：null