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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Rabbits were immunized with T7 cleavage site (MASMTGGQQMG). Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX30555
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, ELISA, IHC
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
T7 tag
- 抗体英文名:
T7 tag antibody
- 抗体名:
T7 tag 抗体
- 规格:
100 μg
ELISA analysis of immunogen using GTX30555 T7 tag antibody.
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文献和实验Gendrel M et al., Nature 2009 (PMID:19794415)
Machado P et al., J Neurosci 2011 (PMID:21209184)
Amorim IS et al., PLoS One 2023 (PMID:37014877)
/L of functional protein. Herein, we report on the production in Escherichia coli and the purification of a recombinant mature streptavidin bearing a T7-tag. Optimization of critical parameters, including the glucose concentration, the pH and the time
使它们得以能进行纯化但这也意味着需要对每一种待纯化的蛋白质研发一套新的方法。所幸的是,尽管存在这种固有的困难,但现已有多种方法可以利用,蛋白质纯化策略也已实际可行。目前,待研究蛋白或酶的基因的获得已是相当普遍的事。可诱导表达系统特别是Studier等发展的以噬菌体T7RNA聚合酶为基础的表达系统的出现使人们能近乎常规地获得过表达(overexpression),表达水平可达细胞蛋白的2%以上,有些甚至高达50%.(一)试剂准备采用T7· Tag Affinity Purification Kit1. T7
%. (一)试剂准备 采用T7· Tag Affinity Purification Kit 1. T7·Tag抗体琼脂 。 2. B/W缓冲液:4.29mM Na2HPO4,1.47 mM KH2PO4,2.7 mM KCl,3. 0.137mM NaCl,1%吐温-20,pH7.3。 4. 洗脱缓冲液: 0.1M柠檬酸,pH2.2。 5. 中和缓冲液:2M Tris,pH10.4. 6. PEG 20000.
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