CD31 (PECAM1) mouse monoclonal

gene_symbol：PECAM1
predicted_size：~100 kDa (endothelium) and ~130 kDa (platelets)
Uniprot ID：P16284
description：CD31 (PECAM1) mouse monoclonal antibody, clone C31.7, Purified
clonality：Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：CD31 (PECAM-1, or platelet endothelial cell adhesion molecule-1) is a surface protein expressed by endothelial cells, monocytes, platelets, granulocytes, and lymphocyte subsets, and makes up a large portion of endothelial intercellular junctions. CD31 is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. Reports indicate that CD31 interacts with CD38 and is involved in cellular interactions resulting in wound healing and angiogenesis. Expression of CD31 on CD4+ T lymphocytes, helps to control T lymphocyte activation, because in the absence of CD31, T cells have a greater propensity to become activated, resulting in increased susceptibility to become apoptotic. This impact of CD31 loss becomes most pronounced during severe, inflammatory, and immunological stresses such as those caused by systemic Salmonella infection. This identifies a novel role for CD31 in regulating CD4 T homeostasis.
immunogen：Human recombinant CD31 protein.
recommended_dilution：ELISA (For coating, Use Antibody without BSA). Western blot: 0.5-1 µg/ml. Immunoprecipitation: 1-2 µg/500 µg protein lysate. Flow Cytometry: 0.5-1 µg/10⁶ cells. Immunofluorescence: 0.5-1 µg/ml.  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 1-2 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.  Positive Control : Jurkat, CCRF-CEM or K-562 Cells. Tonsil, Angiosarcoma.
predicted_size：~100 kDa (endothelium) and ~130 kDa (platelets)
buffer：10mM PBS; State: Purified; State: Liquid purified IgG fraction from Bioreactor Concentrate; Stabilizer: 0.05% BSA; Preservative: 0.05% Sodium Azide
host_species：Mouse
applications：ELISA, FC, IF, IHC, IP, WB
reactivities：Human, Monkey, Rabbit