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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
This monoclonal antibody was developed by immunizing mice with a synthetic peptide containing amino acids 481-497 (CINFTRVKNNQPAKYPL) of human TEM8.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX13798
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
TEM8
- 抗体英文名:
TEM8 antibody [200C1339(SB20)]
- 抗体名:
TEM8 抗体 [200C1339(SB20)]
- 规格:
100 μg
WB analysis of cells overexpressing TEM8 lysate using GTX13798 TEM8 antibody [200C1339(SB20)].
IHC-P analysis of human liver cancer tissue using GTX13798 TEM8 antibody [200C1339(SB20)].
Dilution : 5 μg/ml
WB analysis of human heart tissue lysate (in RIPA buffer) using GTX13798 TEM8 antibody [200C1339(SB20)].
Loading : 35μg
Dilution : 0.3 μg/ml
IHC-P analysis of human colon tissue using GTX13798 TEM8 antibody [200C1339(SB20)].
Dilution : 2.5 μg/ml
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文献和实验whole cells and spheroplasts are used for this purpose. Grow cells in 200 mls of YPD at 30o C to an OD 600 of 1. Add formaldehyde to the 200 ml culture to a final concentration of 3.7%. Fix for 1 hour at room temperature with shaking.
(either undiluted or 1:1 with PBT) or diluted polyclonal antiserum and incubate at 4 C overnight 8. Rinse with PBT 3 times, then wash 4 times for 15 minutes each 9. Add secondary antibody (usually 1:200 in PBT) and incubate 2 hours at room temperature
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
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