MARCO (420-431) mouse monoclon

gene_symbol：MARCO
predicted_size：null
Uniprot ID：Q9UEW3
description：MARCO (420-431) mouse monoclonal antibody, clone PLK-1, Purified
clonality：Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：The scavenger receptors (SRs) expressed by macrophages are thought to play an important role in the immune response against bacteria by mediating ligand binding and phagocytosis. SRs can be divided into three different classes based upon their structural properties, which are termed SR-A, SR-B and SR-C. SRs-A are homotrimeric glycoproteins composed of 77 kDa monomers subdivided into 3 types.. The molecular structure of MARCO resembles that of SR-A type I, containing a triple-helical collagenous domain and a scavenger receptor cysteine-rich (SRCR) domain at the C terminus. MARCO is only expressed in some subpopulations of macrophages, although it’s expression can be strongly upregulated during infection or LPS treatment. Furthermore, MARCO is, like sinusoidal endothelial cell markers DC-SIGNR, LYVE-1 and stabilin-2, expressed by sinusoidal endothelial cells in lymph node. MARCO expressed by alveolar macrophages seems to play an important role in response to inhaled particles and airborne pathogens.
immunogen：Human alveolar macrophages
recommended_dilution：Immunohistochmistry on Frozen Sections: Tissue sections can be  fixed in acetone or 2% paraformaldehyde. PLK-1 antibody was used at 5 µg/ml (Ref.3,4). Flow Cytometry: Antibody PLK-1 weakly stains alveolar macrophages by recognizing the extracellular domain of MARCO. Transfected COS cells were used as positive control (Ref.1). Functional assay: Antibody PLK-1 blocks human alveolar macrophages binding to unopsonized particles (Ref.1,3,5). Immunofluorescence: Aveolar macrophages and transfected CHO cells were stained for MARCO using 0.6 µg/ml PLK-1 antibody (Ref.2,5). Immunoprecipitation: Antibody PLK-1 immunoprecipitates MARCO as 60 and 50 kDa protein from lysates obtained from COS cells transfected with human MARCO (Ref.3). Western blot: Antibody PLK-1 stained MARCO under non-reducing conditions (Ref.6).  Positive Control:  Human alveolar macrophages. Negative Control : All other Human cells.
predicted_size：null
buffer：PBS; State: Purified; State: Liquid 0.2 µm filtered Ig fraction; Stabilizer: 0.1% BSA
host_species：Mouse
applications：FC, FN, IF, IHC, IP, WB
reactivities：Bovine, Human