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- 规格:
500 ug
predicted_size:null
Uniprot ID
description:CA50 mouse monoclonal antibody, clone 93, Aff - Purified
clonality:Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...):Primary Antibodies
background:Cancer antigen 50 (CA50), a carcinoma associated carbohydrate antigen, has been found to be elevated in the blood of patients with colorectal cancer and other malignancies. CA50 has been evaluated in various studies for use as a tumor marker for cancer diagnosis. Results showed that CA50 was marginally useful for the diagnosis and very useful for the follow-up study of patients with pancreatic cancer.
immunogen:Human CA50 protein.
recommended_dilution:ELISA: Use of CA50 antibody (clone 93) at concentration a of 0.2 ng/ml can recognize coated 1 µg/ml of CA50/well. Immunocytochemistry/Immunofluorescence: Metanol-fixed Hela cells were stained successfully with clone 93 at 10 µg/m.
predicted_size:null
buffer:0.01M PBS, pH 7.2 without preservatives.; State: Aff - Purified; State: Lyophilized purified Ig fraction.
host_species:Mouse
applications:ELISA, IF
reactivities:Human
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文献和实验Phycoerythrin conjugation protocol
Phycoerythrin conjugation protocol David's method modified from references (2) and (3). I used this method to conjugate a mouse IgG2a monoclonal antibody (IB4). It works well. Reagents Ab Antibody
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
population is termed monoclonal . It recognizes a single epitope on the antigenic protein. Antibody producing B lymphocytes can be isolated from the spleen or from lymph nodes. However, they have a finite life span in culture, i.e
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