- ￥5973
- OriGene
- AM00063PU-N
- 2026年03月04日
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- 规格：
100 ug
predicted_size：null
Uniprot ID
description：N-Acetylglucosamine (GlcNAc) mouse monoclonal antibody, clone 10D8, Purified
clonality：Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：Many intracellular proteins contain monomeric O - linked N - acetylglucosamine residues conjugated to th hydroxyl group of serine or threonine residues. This modification can interfere with tserine and threonine phosphorylation.
immunogen：N-Acetylglucosamine conjugated to KLH.
recommended_dilution：Western blot: 0.5 μg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.1 - 1.0 µg/ml.
predicted_size：null
buffer：1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose; State: Purified; State: Lyophilized purified IgM
host_species：Mouse
applications：ELISA, WB
reactivities：Human, Mouse, Rat
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
Monoclonal Antibody Production
Monoclonal Antibody Production Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform
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