Matrices listed below were spiked with certain level of TNFRSF13B/TACI and the recovery rates were calculated by comparing the measured value to the expected amount of TNFRSF13B/TACI in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-97
90
EDTA plasma(n=5)
88-104
98
heparin plasma(n=5)
88-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFRSF13B/TACI and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.