Matrices listed below were spiked with certain level of TNFRSF4/OX40 and the recovery rates were calculated by comparing the measured value to the expected amount of TNFRSF4/OX40 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-98
95
EDTA plasma(n=5)
86-94
90
heparin plasma(n=5)
96-104
100
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFRSF4/OX40 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.