pKW08-Lx-Int分枝杆菌表达质粒

基本信息

复制子:	ColE1 ori
终止子:	rrnB T2 terminator，rrnB T1 terminator
质粒分类:	分枝杆菌质粒
质粒大小:	8535bp
质粒标签:	LuxA，LuxB
原核抗性:	潮霉素Hygromycin
克隆菌株:	DH5α
培养条件:	37℃，有氧，LB
表达宿主:	分枝杆菌
5'测序引物:	根据全序列设计
备注:	低拷贝

质粒图谱

质粒序列

LOCUS       Exported                8535 bp ds-DNA     circular SYN 11-SEP-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pKW08-Lx-Int
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, September 11, 2016 from SnapGene Viewer 3.1.4
FEATURES             Location/Qualifiers
     source          1..8535
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(248..836)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      4797..4843
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5617..5633
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      8495..8522
                     /note="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 actagttcta gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
       61 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      121 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      181 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      241 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      301 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      361 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      421 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      481 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      541 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      601 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      661 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
      721 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      781 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
      841 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
      901 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
      961 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     1021 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     1081 ccggacgtgg ccgaccagcc cgtcatcgtc aacgcctgac cgcggtgcgg acaggccgtg
     1141 tcgcgaccgg ccgtgcggaa ttaagccggc ccgtaccctg tgaatagagg tccgctgtga
     1201 cacaagaatc cctgttactt ctcgaccgta ttgattcgga tgattcctac gcgagcctgc
     1261 ggaacgacca ggaattctgg gagccgctgg cccgccgagc cctggaggag ctcgggctgc
     1321 cggtgccgcc ggtgctgcgg gtgcccggcg agagcaccaa ccccgtactg gtcggcgagc
     1381 ccgacccggt gatcaagctg ttcggcgagc actggtgcgg tccggagagc ctcgcgtcgg
     1441 agtcggaggc gtacgcggtc ctggcggacg ccccggtgcc ggtgccccgc ctcctcggcc
     1501 gcggcgagct gcggcccggc accggagcct ggccgtggcc ctacctggtg atgagccgga
     1561 tgaccggcac cacctggcgg tccgcgatgg acggcacgac cgaccggaac gcgctgctcg
     1621 ccctggcccg cgaactcggc cgggtgctcg gccggctgca cagggtgccg ctgaccggga
     1681 acaccgtgct caccccccat tccgaggtct tcccggaact gctgcgggaa cgccgcgcgg
     1741 cgaccgtcga ggaccaccgc gggtggggct acctctcgcc ccggctgctg gaccgcctgg
     1801 aggactggct gccggacgtg gacacgctgc tggccggccg cgaaccccgg ttcgtccacg
     1861 gcgacctgca cgggaccaac atcttcgtgg acctggccgc gaccgaggtc accgggatcg
     1921 tcgacttcac cgacgtctat gcgggagact cccgctacag cctggtgcaa ctgcatctca
     1981 acgccttccg gggcgaccgc gagatcctgg ccgcgctgct cgacggggcg cagtggaagc
     2041 ggaccgagga cttcgcccgc gaactgctcg ccttcacctt cctgcacgac ttcgaggtgt
     2101 tcgaggagac cccgctggat ctctccggct tcaccgatcc ggaggaactg gcgcagttcc
     2161 tctgggggcc gccggacacc gcccccggcg cctgacgccc cgggccgccc ggcgccgccc
     2221 ccggcccccg gcggccgccc ggagccccgc ccgcgctcgg gagccccggg cccgcgccga
     2281 agcccgctgc tgcgagcccg gagcgggccg gccgacggcg gtgcgggccc ggcggcggac
     2341 gctcagcagc ggcgggcgtg aaaggccctg gcatcctcga tcatctcctc cagggtggtc
     2401 ggccgagctt ccatcccagc tcggcaagga tcaacgagga cagtcgcacg acgaagttct
     2461 tctggatcgc gcccgtgctg gaagcactca acctcgaagc gtgtggttgc ggagccatct
     2521 agcaaccaca cgaaacatgc gcaacgaacc gcgcaacgaa caacgcctag aactggccct
     2581 agatgagctg actcgtatcg ttggtaaacc tagtttgacc agcatgtttt aactacgttc
     2641 ggtgagctgt caacggggcc tgtaacggca caacgaaccg tgcaacgaga gtggccacgg
     2701 atgccaccac aggcactaca acggagttcg ccacgtacat caccacaacc accgattctg
     2761 gcggtgagct ccccgatatt cagcggaaat ggcttggtat cgaccaagat tcgtagaacc
     2821 ccgtctcgtc tggctggtat tcaaaacggg cgcaacgaaa cacgcaacga gacaggcatg
     2881 gcccaaacca gaaaactagc gtctaccagg acttttacct gtccgacccg ttgcaacgga
     2941 accccccacg gaacccccgc gacacccgct ccccaattgt tgctagctgg cgggaacggc
     3001 aatgctcctg gcggcggcgg ggctggtgcc caggtctccg cacagagcgg cggtgctggc
     3061 gctcgcggcc aggcgtggtt cttcgcgtac tgacaagaaa cccccctctt taggactcag
     3121 tgtccttggg aggggggctt tttgcgtttc aggaggtctt ggccagcttg gacatcgcct
     3181 cagcgatagc ctcgtcgcgg gcctcagacg ccatctggta cttcatcgcc atcctaggag
     3241 tcgtgtgacc gagacgggcc atcagctcct tggtcgtcgc acctgcctga gcggcgaacg
     3301 tagcgccgac agcgcggagg tcgtggatgc ggagttccgg ccgaccgatc ttggcgtagc
     3361 cacgcttcag cgacttggtg aacgcggact tcgacagccg gttgccctgc gtcgtggtca
     3421 ccaggaatgc ctcggggccc ttgttcatct tcgtacggtc cttcatgtgc gctcggatca
     3481 tctccgcgac gtgaggcgga accgtcacag gacgcttcga ccggacggtc ttggcgttgc
     3541 caacgacgat cttgttcccc acgcgggaag cgccacggcg cacccggagc ttcatcgtca
     3601 tgccgtcgtc cacgatgtcc ttgcggcgaa gctcgatcag ctctccgaac cggaggctcg
     3661 tccacgccag gatgtatgcc gcgatccggt agtgctcgaa gatctcagcg gcgacgatgt
     3721 ccagctcctc aggcgtcagc gcctctacgt cgcgctcatc ggctgccttc tgctcgatcc
     3781 ggcacgggtt ctctgcgatc agcttgtcct cgaccgctgt gttcatcacc gcccggagga
     3841 cgttgtaggc atgccggcgg gcagtcgggt gcttcctacc catcccggcc caccacgcac
     3901 gcaccagagc tggcgtcatc tctgtgaccg ccacttcacc tagcaccggg tagatgcggc
     3961 gctccgcgtg cccgctgtac agatccctgg tgccgtctgc gaggtcgcgc tccacgagcc
     4021 acttccgggt gtactcctcc agcgtgatgg cgctggcggc tgccttcttc gcccggtcct
     4081 gtggaggggt ccaggtctcc atctcgatga gccgcttctc gcccgcgagc caggcttcgg
     4141 cgtccatctt gttgtcgtag gtctgcagcg cgtagtacct cacaccgtcc tgcgggttga
     4201 cgtatgaggc ttggatcctc ccgctgcgct gagtcttcag cgatccccat ccgcgacgtg
     4261 ccaactaggt ctcctctcgt cgtgaacaag gctaccgggt tgcaactcct gtgcaactct
     4321 caggcttcaa cgcgcttcta cgacctgcaa tttctttcca cttagaggat gcagccgaga
     4381 gggggtaaaa acctatcttg accggcccat atatgtggtc ggcagacacc cattcttcca
     4441 aactagctac gcgggttcga ttcccgtcgc ccgctccgct ggtcagaggg tgttttcgcc
     4501 ctctggccat ttttctttcc aggggtctgc aactcttgtg cgactcttct gacctgggca
     4561 tacgcggttg caacgcatcc ctgatctggc tactttcgat gctgacaaac gaatagagcc
     4621 ccccgcctgc gcgaacagac gaggggcatt cacaccagat tggagctggt gcagtgaaga
     4681 gaatagaccg ggacaaggtt gcaccgggag ttgcagcggt cggaaccctc gccgtcggcg
     4741 ggctggcgtt cgccctgtcg ttcacggctc tagctgatca ccgcggccat gatggcataa
     4801 aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg gcagtcgatc
     4861 gtacaccggt gtatggtccg acacgagctc caagtcagat atttcgctga ggggccaccc
     4921 cacaactgca cactcccccg ctctcccgtc gagccctggt ggtggaacac cagcgacagc
     4981 cgagcacccc caaccacctg taccaaccag gaggaacaca tgcgtcgttt cgaggacgtt
     5041 tccgggccgc tgagagccgc tgtggcggcc gtacacgccg ccttagaccc gttagacccc
     5101 ctgccgcctg aatgcgcggg tacgagccac acagcgcccg aacttacgga gctggtgggc
     5161 tcacctggct ttatggcgta cgaatcggct gtgtgcgacc tgttgggcga ggtgaggtac
     5221 gcgctactca cgctggcaag ggcgacacag ccgccccacc gagcccgcac ggccgcgcgc
     5281 ggtgtcaaca accgggtgag tcgtgcacac cagcaggtgt tcgaggcttg gctcgaagtg
     5341 caggacatcg tggcgaacgc cgcccgatga gccgcgcctt acgctggctg ccagccgttc
     5401 gcgggctggt tggtgcagcg cgtcgagcgg ttagaggccc tgcggtgttc caccaccgca
     5461 ggcctcgccc tttttaaggc tgaatttgct tgtctccgaa tccaactggc ttgtccaagg
     5521 gtgtatctac gcttaatcca aagttcaaac gaggggatta cacatgacca acttcgataa
     5581 cgttctcggc tcgatgatcc ccggcgggcc ccccctcgag gtcgacggta tcgataaaat
     5641 aaaaaagggg acctctaggg tccccaatta attagctaaa gcttttacga gtggtatttg
     5701 acgatgttgg cgtttaccac atcgataacc gctctttgct gcgctttatc ttccatcgac
     5761 tcaaaagaca tcaataggtc cgcggcacca caacactcaa tcgcaactcg cgctgcctga
     5821 gtactttctt cataagtacc gatggcattt tctgacaaca gctctcccat tttttgctca
     5881 aagtcggtat ttgagtaaga ttcacggaca aactcttcca aatacacgcg agcttctgcc
     5941 cttgctgctt caccatctac attttggttg accagcagcg tcagcttgtg tcgaacctga
     6001 ctaacatcga caccatgtgc ctgagcaact tcgtggtaca aaccggcgta ttcttttctt
     6061 tgagcgtttg agtcgtccca tctaaacacg agtggaagcc ctaacttagc cgcccattca
     6121 accacttctt tgctcgtcgc attcacaaat tgcgcaggac cgccttcagt gaacgcgtgt
     6181 gggttaacgg agattttagg aaaactataa aaatcattgt ttggatggca gtacccagta
     6241 gtgaatgaat cattgatgat cttgtgacac tcactgaaca actgaaactg agaatccgtt
     6301 ggtcgattaa agaagcgcat atctgcactc ttttcacaat cactaaagcc aaaggcaaaa
     6361 cggccttcac tcatttggtc aagtagacac gcttcttccg ccacacgtac tggatgatgc
     6421 gtggtaatga cgtgattcaa cgaagccact ttggcgttct ttgtcatacc aagtaaaaaa
     6481 ccagccactg ttagtggggc accaaccaca ccattgttcg agaaatggtt ttcgtaaaca
     6541 gccaacgtgt caaacttcaa ctgatctacg taatgtgcgg tatctaacat ttcttcgatg
     6601 acttgatcag aagaacgctt tgagttcata aaattgagga agaataatcc aaatttcatg
     6661 tctctttcct tttagaaaat attaattact gtttttcttt gagatatggc atcacatcag
     6721 actggaatag cttcatagat gcgataattt cttcttcaga accgtttgct tcaaaaccac
     6781 aacaaatatt gtcaataccc gtcgcatcaa tatcttgctg gataatcgcg atacactctt
     6841 caggcgtccc tactgggttg atttcgtagc tgtaatcaat tcggcgattg gtgtctttgt
     6901 ggcctttcaa cacaaaatca cgccattgac ctttattgaa gtcgtaacct tttgtttggt
     6961 cagagtcgtc aaaaatcttg gtggcattca cgtatgagtc gtaccaatgg cccaagaagt
     7021 tgcggcaaat atctttggct ctatttgagt catgatcgac ggaggtgatg taagacaaac
     7081 agtggtcaat cttagtcaca tcgtagccat gttcagtcgc gacttcgttg taaagatcaa
     7141 gctgcgcttt cttctcgtga gtgttgatga tccagcttag aatcattggt aggccacgct
     7201 ctgcagccca ttctgtcgtt gatgctgact ccgcgacgac ataaacagga gcaccacctt
     7261 gtgtgtaagc cgatggattc agttggattt tcgggaactt aatatgttcg ttatccgccg
     7321 cgatatagcc ttcattgaag ccttctttca tcaagtcata ccaacagtcc attaaggctc
     7381 ggctgttatc catgtctgta ccaaagacac gaaaatcttt atcgtacaaa ccgcgacaaa
     7441 taccaaaacg gaatcgtcct tttgacattt gatccagtag gtttacgtct tctgcttgtc
     7501 gaaccggatg ggcagtcggc aatacgatag ctgcagtgcc aacgttgagc gtttctgtcg
     7561 cacctaatag gtgtgcggca gcaacataag gattccctaa caacccaaat tcagtgaagt
     7621 ggtgctctag caaccaaacg gtgtcgaagc cacaaccttc agacgctttg cccagattaa
     7681 ccaatcgctt catcacttcg gtctgagata gctcaggtgg ctgataagtg aggaggaagt
     7741 ttccaaattt cataacattt cctccggatc ctgtcaggat tccacgatga gagtagcgag
     7801 cagtcagatg agagtagcga gcagtcactt tatctaagat aaagttatcg atgataaagt
     7861 gcaactatga ctcaccaccg gaagcgtctc gaccgcacgt ccgtgttgct cggggcccgg
     7921 caggtgctcg acgagactgg gctcgatggc ttcaccactc gcgcgctggc ggctcactta
     7981 caggtacagc agcccggcct ctactggcac ttccgcacga aggccgaact cctcgcagcg
     8041 ctggcgagcg acgtgcttga ccgagagcat cacgcttcct tccccgagtc cgacgagaaa
     8101 tgggacgcat ttctcttgcg aaacgcaaga agtttccgcc acgcactaca cgcggtccgt
     8161 gacggtgcgc gtctccacgc tgagcaccac cgcagaccat ccggcgacga caccgacgaa
     8221 ggagctgagg cttccggtca gcaagtcggg cttctcgtgt ctcagggatt cgatgaacag
     8281 acagccatca gcatgctcat cgcggtcagc cgatacaccg tcggtgtcgt actggaggag
     8341 caaaccgcag aggtcggcaa tgcgagcgtt cctgaacgcg accgggaatt cgatttcggc
     8401 ctgacagcac tgatcgacgg attctctcac tcccgcacag cgtcattgac ctcgagcgaa
     8461 tcgtgatcta gaggtttaaa cagtattaaa cgcaaaaagg ccatccgtca ggatggcctt
     8521 ctgcttagct aatca
//