pMA5枯草胞内质粒

基本信息

启动子:	HpaII promoter
复制子:	ColE1 Ori，F1 Ori
质粒分类:	枯草杆菌质粒；枯草游离质粒；枯草胞内质粒
质粒大小:	7202bp
原核抗性:	氨苄青霉素Amp
克隆菌株:	DH5α等大肠杆菌
培养条件:	37℃，有氧，LB

质粒简介

        pMA5质粒是一个枯草芽孢杆菌表达载体，HpaII promoter驱动目的基因的表达。

质粒图谱

质粒序列

LOCUS       Exported                7202 bp ds-DNA    circular SYN 08-九月-2017
KEYWORDS    pMA5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7202)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      429..884
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1006..1110
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             1111..1971
                     /codon_start=1
                     /note="Amp"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2142..2733
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5317..6078
                     /note="Kan"
     misc_feature    6934..3
                     /note="HpaII promoter"
ORIGIN
        1 catatgagtt atgcagtttg tagaatgcaa aaagtgaaat cagggggatc ctctagagtc
       61 gagctcaagc tagcttggta cgtaccagat ctgagatcac gcgttctaga ggtcgaaatt
      121 cacctcgaaa gcaagctgat aaaccgatac aattaaaggc tccttttgga gccttttttt
      181 ttggagattt tcaacgtgaa aaaattatta ttcgcaattc caagctaatt cacctcgaaa
      241 gcaagctgat aaaccgatac aattaaaggc tccttttgga gccttttttt ttggagattt
      301 tcaacgtgaa aaaattatta ttcgcaattc caagctctgc ctcgcgcgtt tcggtgatga
      361 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
      421 tgcagatcac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
      481 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
      541 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
      601 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
      661 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
      721 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
      781 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
      841 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttgatctg cgctcggtcg
      901 ttcggctgcg gcgagcggta tcagctcact caaagcggta atacggttat cccagaatcc
      961 ggggataacg caggaaagac aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1021 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1081 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1141 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1201 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1261 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1321 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     1381 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1441 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1501 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1561 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1621 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
     1681 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     1741 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     1801 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     1861 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     1921 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     1981 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2041 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2101 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2161 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2221 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2281 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2341 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2401 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2461 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga agtactgaga
     2521 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2581 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2641 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2701 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2761 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     2821 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     2881 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     2941 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     3001 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     3061 cactttatgc ttccggcata ttctcaataa accctttagg gaaataggcc aggttttcac
     3121 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     3181 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     3241 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat
     3301 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     3361 cggtctttaa aaaggccgta atatccagct aaacggtctg gttataggta cattgagcaa
     3421 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     3481 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     3541 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     3601 gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac
     3661 caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg aagacgaaag
     3721 ggcatcgcgc gcggggaatt cccgggagag ctcgatatcg catgcggtac ctctagaaga
     3781 agcttggaga caaggtaaag gataaaacag cacaattcca agaaaaacac gatttagaac
     3841 ctaaaaagaa cgaatttgaa ctaactcata accgagaggt aaaaaaagaa cgaagtcgag
     3901 atcagggaat gagtttataa aataaaaaaa gcacctgaaa aggtgtcttt ttttgatggt
     3961 tttgaacttg ttctttctta tcttgataca tatagaaata acgtcatttt tattttagtt
     4021 gctgaaaggt gcgttgaagt gttggtatgt atgtgtttta aagtattgaa aacccttaaa
     4081 attggttgca cagaaaaacc ccatctgtta aagttataag tgactaaaca aataactaaa
     4141 tagatggggg tttcttttaa tattatgtgt cctaatagta gcatttattc agatgaaaaa
     4201 tcaagggttt tagtggacaa gacaaaaagt ggaaaagtga gaccatggag agaaaagaaa
     4261 atcgctaatg ttgattactt tgaacttctg catattcttg aatttaaaaa ggctgaaaga
     4321 gtaaaagatt gtgctgaaat attagagtat aaacaaaatc gtgaaacagg cgaaagaaag
     4381 ttgtatcgag tgtggttttg taaatccagg ctttgtccaa tgtgcaactg gaggagagca
     4441 atgaaacatg gcattcagtc acaaaaggtt gttgctgaag ttattaaaca aaagccaaca
     4501 gttcgttggt tgtttctcac attaacagtt aaaaatgttt atgatggcga agaattaaat
     4561 aagagtttgt cagatatggc tcaaggattt cgccgaatga tgcaatataa aaaaattaat
     4621 aaaaatcttg ttggttttat gcgtgcaacg gaagtgacaa taaataataa agataattct
     4681 tataatcagc acatgcatgt attggtatgt gtggaaccaa cttattttaa gaatacagaa
     4741 aactacgtga atcaaaaaca atggattcaa ttttggaaaa aggcaatgaa attagactat
     4801 gatccaaatg taaaagttca aatgattcga ccgaaaaata aatataaatc ggatatacaa
     4861 tcggcaattg acgaaactgc aaaatatcct gtaaaggata cggattttat gaccgatgat
     4921 gaagaaaaga atttgaaacg tttgtctgat ttggaggaag gtttacaccg taaaaggtta
     4981 atctcctatg gtggtttgtt aaaagaaata cataaaaaat taaaccttga tgacacagaa
     5041 gaaggcgatt tgattcatac agatgatgac gaaaaagccg atgaagatgg attttctatt
     5101 attgcaatgt ggaattggga acggaaaaat tattttatta aagagtagtt caacaaacgg
     5161 gccagtttgt tgaagattag atgctataat tgttattaaa aggattgaag gatgcttagg
     5221 aagacgagtt attaatagct gaataagaac ggtgctctcc aaatattctt atttagaaaa
     5281 gcaaatctaa aattatctga aaagggaatg agaatagtga atggaccaat aataatgact
     5341 agagaagaaa gaatgaagat tgttcatgaa attaaggaac gaatattgga taaatatggg
     5401 gatgatgtta aggctattgg tgtttatggc tctcttggtc gtcagactga tgggccctat
     5461 tcggatattg agatgatgtg tgtcatgtca acagaggaag cagagttcag ccatgaatgg
     5521 acaaccggtg agtggaaggt ggaagtgaat tttgatagcg aagagattct actagattat
     5581 gcatctcagg tggaatcaga ttggccgctt acacatggtc aatttttctc tattttgccg
     5641 atttatgatt caggtggata cttagagaaa gtgtatcaaa ctgctaaatc ggtagaagcc
     5701 caaacgttcc acgatgcgat ttgtgccctt atcgtagaag agctgtttga atatgcaggc
     5761 aaatggcgta atattcgtgt gcaaggaccg acaacatttc taccatcctt gactgtacag
     5821 gtagcaatgg caggtgccat gttgattggt ctgcatcatc gcatctgtta tacgacgagc
     5881 gcttcggtct taactgaagc agttaagcaa tcagatcttc cttcaggtta tgaccatctg
     5941 tgccagttcg taatgtctgg tcaactttcc gactctgaga aacttctgga atcgctagag
     6001 aatttctgga atgggattca ggagtggaca gaacgacacg gatatatagt ggatgtgtca
     6061 aaacgcatac cattttgaac gatgacctct aataattgtt aatcatgttg gttacgtatt
     6121 tattaacttc tcctagtatt agtaattatc atggctgtca tggcgcatta acggaataaa
     6181 gggtgtgctt aaatcgggcc attttgcgta ataagaaaaa ggattaatta tgagcgaatt
     6241 gaattaataa taaggtaata gatttacatt agaaaatgaa aggggatttt atgcgtgaga
     6301 atgttacagt ctatcccggc attgccagtc ggggatatta aaaagagtat aggtttttat
     6361 tgcgataaac taggtttcac tttggttcac catgaagatg gattcgcagt tctaatgtgt
     6421 aatgaggttc ggattcatct atgggaggca agtgatgaag gctggcgctc tcgtagtaat
     6481 gattcaccgg tttgtacagg tgcggagtcg tttattgctg gtactgctag ttgccgcatt
     6541 gaagtagagg gaattgatga attatatcaa catattaagc ctttgggcat tttgcacccc
     6601 aatacatcat taaaagatca gtggtgggat gaacgagact ttgcagtaat tgatcccgac
     6661 aacaatttga ttagcttttt tcaacaaata aaaagctaaa atctattatt aatctgttca
     6721 gcaatcgggc gcgattgctg aataaaagat acgagagacc tctcttgtat cttttttatt
     6781 ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt tattcttgct
     6841 gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca agggtttaaa
     6901 ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc tgatttttaa
     6961 acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt tttttgtttc
     7021 ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc ggcactgccg
     7081 acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata ctttacttgg
     7141 aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt
     7201 ta