NGFR人源基因质粒

质粒图谱

质粒序列

LOCUS       Exported                6521 bp ds-DNA     circular SYN 28-SEP-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    NGFR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 28, 2017 from Plasmid
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(681..1541)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1542..1646)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             2474..2990
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    3054..3395
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             3462..3878
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     misc_feature    3923..4475
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4487..5289
                     /label=NGFR
                     /note="Homo sapiens nerve growth factor receptor (NGFR), 
                     mRNANCBI Reference Sequence: NM_002507.3"
     LTR             5389..5903
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6065..6081
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6089..6119)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6134..6155
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6443..510)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
       61 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      121 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      181 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      241 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      301 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      361 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      421 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      481 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      541 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      601 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
      661 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
      721 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
      781 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
      841 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
      901 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
      961 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     1021 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     1081 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     1141 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     1201 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     1261 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     1321 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     1381 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     1441 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     1501 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     1561 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     1621 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     1681 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     1741 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     1801 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     1861 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     1921 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     1981 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     2041 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     2101 ttttcccagt cacgacgttg taaaacgacg gcgcaaggaa tggtgcatgc aaggagatgg
     2161 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     2221 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     2281 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggcgattag
     2341 tccaatttgt taaagacagg atatcagtgg tccaggctct agttttgact caacaatatc
     2401 accagctgaa gcctatagag tacgagccat agataaaata aaagatttta tttagtctcc
     2461 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     2521 cattttgcaa ggcatggaaa atacataact gagaatagag aagttcagat caaggttagg
     2581 aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     2641 ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagag
     2701 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     2761 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     2821 aagagcccac aacccctcac tcggcgcgcc agtcctccga tagactgcgt cgcccgggta
     2881 cccgtattcc caataaagcc tcttgctgtt tgcatccgaa tcgtggactc gctgatcctt
     2941 gggagggtct cctcagattg attgactgcc cacctcgggg gtctttcatt tggaggttcc
     3001 accgagattt ggagacccct gcccagggac caccgacccc cccgccggga ggtaagctgg
     3061 ccagcggtcg tttcgtgtct gtctctgtct ttgtgcgtgt ttgtgccggc atctaatgtt
     3121 tgcgcctgcg tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
     3181 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
     3241 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
     3301 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
     3361 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
     3421 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
     3481 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     3541 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     3601 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     3661 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     3721 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     3781 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     3841 cctcccttta tccagccctc actccttctc taggcgccgg aattagatct ctcgaggtta
     3901 acgaattccg cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     3961 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     4021 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     4081 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     4141 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     4201 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     4261 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     4321 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     4381 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     4441 gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg acgggccgcg
     4501 cctgctgctg ttgctgcttc tgggggtgtc ccttggaggt gccaaggagg catgccccac
     4561 aggcctgtac acacacagcg gtgagtgctg caaagcctgc aacctgggcg agggtgtggc
     4621 ccagccttgt ggagccaacc agaccgtgtg tgagccctgc ctggacagcg tgacgttctc
     4681 cgacgtggtg agcgcgaccg agccgtgcaa gccgtgcacc gagtgcgtgg ggctccagag
     4741 catgtcggcg ccgtgcgtgg aggccgacga cgccgtgtgc cgctgcgcct acggctacta
     4801 ccaggatgag acgactgggc gctgcgaggc gtgccgcgtg tgcgaggcgg gctcgggcct
     4861 cgtgttctcc tgccaggaca agcagaacac cgtgtgcgag gagtgccccg acggcacgta
     4921 ttccgacgag gccaaccacg tggacccgtg cctgccctgc accgtgtgcg aggacaccga
     4981 gcgccagctc cgcgagtgca cacgctgggc cgacgccgag tgcgaggaga tccctggccg
     5041 ttggattaca cggtccacac ccccagaggg ctcggacagc acagccccca gcacccagga
     5101 gcctgaggca cctccagaac aagacctcat agccagcacg gtggcaggtg tggtgaccac
     5161 agtgatgggc agctcccagc ccgtggtgac ccgaggcacc accgacaacc tcatccctgt
     5221 ctattgctcc atcctggctg ctgtggttgt gggccttgtg gcctacatag ccttcaagag
     5281 gtggaacagt catcgatatc ctcgaggtca ccgcggtcta gagtcgacct gcagccaagc
     5341 ttatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
     5401 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaatacat
     5461 aactgagaat agagaagttc agatcaaggt taggaacaga gagacagcag aatatgggcc
     5521 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc
     5581 ccagatgcgg tcccgccctc agcagtttct agagaaccat cagatgtttc cagggtgccc
     5641 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt
     5701 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg
     5761 cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg tatccaataa accctcttgc
     5821 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact
     5881 acccgtcagc gggggtcttt catgggtaac agtttcttga agttggagaa caacattctg
     5941 agggtaggag tcgaatatta agtaatcctg actcaattag ccactgtttt gaatccacat
     6001 actccaatac tcctgaaata gttcattatg gacagcgcag aaagagctgg ggagaattgt
     6061 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     6121 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     6181 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     6241 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     6301 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     6361 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6421 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6481 atcgacgctc aagtcagagg tggcgaaacc cgacaggact a