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- 详细信息
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- 技术资料
- 保存条件：
负20度
- 保质期：
2年
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钦诚生物
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|---|---|---|---|
| 规格： | 5UG | 产品价格： | ¥1500.0 |
质粒图谱
质粒序列
LOCUS Exported 5254 bp ds-DNA circular SYN 15-MAR-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5254)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 28, 2017 from Plasmid
FEATURES Location/Qualifiers
source 1..5254
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 59..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 613..1329
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
misc_feature 1330..1918
/label=MCS
/note="multiple cloning site of fluorescent protein
plasmids"
CDS 1351..1881
/codon_start=1
/product="Homo sapiens CD274 molecule (CD274), transcript
variant 2, mRNA
NCBI Reference Sequence: NM_001267706.1"
/label=PDL1
/translation="MRIFAVFIFMTYWHLLNAPYNKINQRILVVDPVTSEHELTCQAEG
YPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEE
NHTAELVIPELPLAHPPNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDT
NSKKQSDTHLEET"
polyA_signal 2042..2163
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2170..2625)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2652..2756
/gene="bla"
/label=AmpR promoter
promoter 2758..3115
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2966..3101
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3150..3944
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 4176..4223
/label=HSV TK poly(A) signal
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 4552..5140
/direction=RIGHT
/label=ori
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
1321 ctgtacaagt ccggactcag atctcgagct atgaggatat ttgctgtctt tatattcatg
1381 acctactggc atttgctgaa cgccccatac aacaaaatca accaaagaat tttggttgtg
1441 gatccagtca cctctgaaca tgaactgaca tgtcaggctg agggctaccc caaggccgaa
1501 gtcatctgga caagcagtga ccatcaagtc ctgagtggta agaccaccac caccaattcc
1561 aagagagagg agaagctttt caatgtgacc agcacactga gaatcaacac aacaactaat
1621 gagattttct actgcacttt taggagatta gatcctgagg aaaaccatac agctgaattg
1681 gtcatcccag aactacctct ggcacatcct ccaaatgaaa ggactcactt ggtaattctg
1741 ggagccatct tattatgcct tggtgtagca ctgacattca tcttccgttt aagaaaaggg
1801 agaatgatgg atgtgaaaaa atgtggcatc caagatacaa actcaaagaa gcaaagtgat
1861 acacatttgg aggagacgtg agaattctgc agtcgacggt accgcgggcc cgggatccac
1921 cggatctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt
1981 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
2041 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
2101 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
2161 ttaacgcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
2221 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
2281 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
2341 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
2401 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
2461 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
2521 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
2581 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
2641 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
2701 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg
2761 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
2821 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
2881 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
2941 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
3001 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
3061 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg
3121 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
3181 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
3241 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
3301 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
3361 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
3421 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
3481 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
3541 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
3601 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
3661 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
3721 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
3781 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
3841 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
3901 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
3961 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
4021 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
4081 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg
4141 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
4201 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
4261 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
4321 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
4381 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt
4441 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
4501 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
4561 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
4621 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
4681 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
4741 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
4801 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
4861 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
4921 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
4981 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
5041 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
5101 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
5161 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
5221 tgattctgtg gataaccgta ttaccgccat gcat
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文献和实验相关实验
Restriction Map and Multiple Cloning Site of pEGFP-N2. (Unique restriction sites are in color or bold.) The Not I site follows the EGFP stop codon. The Nhe I site cannot be used for fusions since it contains an in-frame stop codon
多克隆位点区 Restriction Map and Multiple Cloning Site of pEGFP-N1. (Unique restriction sites are in color or bold.) The Not I site follows the EGFP stop codon. The Xba I site (*) is methylated in the DNA provided by CLONTECH. If you wish to digest
多克隆位点区（MCS） Restriction Map and Multiple Cloning Site of pEGFP-C2. (Unique restriction sites are in color or bold.) Note that the Eag I site is not unique.) The Xba I site cannot be used for fusions
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pEGFP-PDL1人源基因质粒
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