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- 规格：
200 ug
predicted_size：null
Uniprot ID：P8174
description：CD55 / DAF mouse monoclonal antibody, clone BRIC216, Purified
clonality：Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：CD55 antigen is a 70 kD glycoprotein distributed on erythrocytes and other circulating blood cells and also on cells in non-haemopoietic tissue particularly epithelium and endothelium. Found specifically at the foetal-maternal interfaces in placenta. CD55 has reduced expression on individuals with paroxysmal noctural haemoglobinuria.
immunogen：Human fibroblast cell line.
recommended_dilution：Flow Cytometry. Immunoprecipitation. Has been described for previous lots to work in Western blotting under non-reducing conditions.
predicted_size：null
buffer：State: Purified; State: Liquid purifed IgG fraction with 0.09% Sodium Azide as preservative.
host_species：Mouse
applications：FC, IP, WB
reactivities：Human
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文献和实验The production of monoclonal antibody by hybridoma
, A., Popkov, M., Cortez, X., Fuller, R., Elia, M., Kerwin, L., and C.F. Barbas III. (2003) Rapid monoclonal antibody generation via dendritic cell targeting in vivo. Hybridoma and Hybridomics 22 (1), 23-31.
Phycoerythrin conjugation protocol
Phycoerythrin conjugation protocol David's method modified from references (2) and (3). I used this method to conjugate a mouse IgG2a monoclonal antibody (IB4). It works well. Reagents Ab Antibody
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
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