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2 ml
predicted_size:null
Uniprot ID:P112
description:OVAL rabbit polyclonal antibody, Serum
clonality:Polyclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...):Primary Antibodies
background:Chicken Ovalbumin is the major protein in the "white" of the egg (and a favorite antigen in immunological research). Egg white contains a variety of proteins including ovalbumin, conalbumin, ovomucoid and lysozyme. It belongs to the serpin family and the Ov serpin subfamily. Ovalbumin can cause an allergic reaction in humans.
Ovalbumin has been implicated in the development of the egg shell. Immunohistochemistry revealed that ovalbumin is found only in the mammillary bodies of decalcified shell, and is not distributed throughout the shell matrix. These results indicate that ovalbumin is present during the initial phase of shell formation and becomes incorporated into the protein matrix of the mammillary bodies. However, it is not yet clear whether ovalbumin at this site plays a specific role in shell mineralisation.
Anti Ovalbumin Antibody recognizes ovalbumin that is the main protein found in egg white. Ovalbumin makes up 60-65% of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a serine protease inhibitor. The function of ovalbumin is unknown, although it is presumed to be a storage protein.
immunogen:Ovalbumin from Hen Egg White. Anti-Ovalbumin Antibody was produced by repeated immunizations with hen egg white Ovalbumin protein.
recommended_dilution:Suitable for: Western blot: 1/500-1/5,000 ELISA: 1/5,000-1/20,000 Immunoprecipitation: 1/100 Recommended Dilutions : This product has been assayed against 1.0 µg of Ovalbumin [Hen Egg White] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (Cat#R1364HRP) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/90,000 to 1/450,000 of the reconstitution concentration is suggested for this product.
predicted_size:null
buffer:0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2; State: Serum; State: Lyophilized Antiserum; Stabilizer: None; Preservative: 0.01% (w/v) Sodium Azide
host_species:Rabbit
applications:ELISA, IP, WB
reactivities:Chicken
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文献和实验Polyclonal Antibody Production
to the pre-bleed, but get at least 20 mL per rabbit, with 40 mL being the upper limit for an adult rabbit (any more and they get anemic). Monitoring of Titer The quality of the antibodies in serum (humoral immune response) of the bleeds
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
Construction of Polyclonal Antibody Libraries Using Phage Display
Polyclonal antibody libraries (PCALs) are standardized mixtures of antibodies (Abs) specific for an antigen (Ag) or multi-Ag target (a poly-Ag). As the immunoglobulin (Ig) genes are cloned, the mixtures can be perpetuated, amplified
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