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- OriGene
- DP3516P
- 2025年07月15日
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- 技术资料
- 规格：
50 ug
predicted_size：null
Uniprot ID：Q92786
description：PROX1 (C-term) rabbit polyclonal antibody, Aff - Purified
clonality：Polyclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：Prox-1 is a homeobox gene and acts as a master switch for lymphatic endothelial phenotype. Expression of Prox-1 in blood endothelial cells induces expression of other lymphatic marker genes. Together with Podoplanin, Prox-1 can be used to reliably distinguish lymphatic vessels from blood vessels. Prox-1 is expressed in CNS, eye, pancreas, liver and heart, and it is one of the most specific and reliable markers for lymphatic endothelial cells.
immunogen：Recombinant Human C-terminal part of the Homeobox Transcription Factor Prox-1 (homeo domain and prospero domain). (Cat.-No DA3565)
recommended_dilution：Immunofluorescence: Use 1/750-1/1000 dilution and 1 µg/ml on cells. Western Bloting: Use 1-5 µg/ml. Immunohistochemistry.
predicted_size：null
buffer：5mM PBS, pH 7.2 without preservatives; State: Aff - Purified; State: Lyophilized purified IgG fraction
host_species：Rabbit
applications：ELISA, IF, IHC, WB
reactivities：Human, Mouse, Rat
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文献和实验Use of EIA to Measure MMPs and TIMPs
the combination of a monoclonal antibody as the solid-phase with rabbit polyclonal antibody and horseradish peroxidase (HRP)-labeled donkey antirabbit gamma globulin as the second phase (2 ). EIAs for human MMP-9 were developed using MMP-9 specific polyclonal
Tips and hints for the storage of antibodies
! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses. Rabbit antisera Antisera are more robust than monoclonals. With anti-microbials added, they may be stored at ℃. Serum does not contain active proteases
Immunocytology using the HA epitope
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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