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- 规格:
100 ul
predicted_size:27 kDa
Uniprot ID
description:Mouse monoclonal turboYFP antibody, clone OTI10F11
clonality:Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...):Tag Antibodies
background:null
immunogen:Full length tYFP protein expressed in HEK293T cells
recommended_dilution:WB: 1:2000, IF: 1:50, FC: 1:400
predicted_size:27 kDa
buffer:null
host_species:Mouse
applications:FC, IF, WB
reactivities:tYFP
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production
Monoclonal Antibody Production Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
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