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- 规格：
100 ug
predicted_size：58.4 kDa
Uniprot ID：Q7Z4H8
description：Carrier-free (BSA/glycerol-free) KDELC2 mouse monoclonal antibody,clone OTI5G2
clonality：Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：null
immunogen：Full length human recombinant protein of human KDELC2 (NP_714916) produced in HEK293T cell.
recommended_dilution：WB 1:500~2000, IHC 1:150
predicted_size：58.4 kDa
buffer：Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
host_species：Mouse
applications：IHC, WB
reactivities：Human
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文献和实验Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Monoclonal Antibody Production
Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
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