Matrices listed below were spiked with certain level of NT-proBNP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-proBNP in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
92
EDTA plasma(n=5)
90-104
98
heparin plasma(n=5)
93-100
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-proBNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.