Matrices listed below were spiked with certain level of MBP/MBL and the recovery rates were calculated by comparing the measured value to the expected amount of MBP/MBL in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
96
EDTA plasma(n=5)
87-99
92
heparin plasma(n=5)
91-103
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MBP/MBL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.