Matrices listed below were spiked with certain level of IL7R and the recovery rates were calculated by comparing the measured value to the expected amount of IL7R in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-105
94
EDTA plasma(n=5)
87-103
95
heparin plasma(n=5)
88-101
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL7R and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.