Matrices listed below were spiked with certain level of CTSL2 and the recovery rates were calculated by comparing the measured value to the expected amount of CTSL2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-97
90
EDTA plasma(n=5)
85-101
92
heparin plasma(n=5)
90-101
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CTSL2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.