Matrices listed below were spiked with certain level of Cortisol and the recovery rates were calculated by comparing the measured value to the expected amount of Cortisol in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-104
95
EDTA plasma(n=5)
86-104
95
heparin plasma(n=5)
86-103
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cortisol and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.