Matrices listed below were spiked with certain level of GPT2 and the recovery rates were calculated by comparing the measured value to the expected amount of GPT2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-101
96
EDTA plasma(n=5)
85-97
90
heparin plasma(n=5)
86-99
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GPT2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.