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文献和实验MEDIA FOR EMBRYO CULTURE AND MANIPULATION
Ingredient % of Final Volume 1 x DMEM with 20mM HEPES buffer 80.8% Penicillin/Streptomycin (5,000 i.u/ml/5,000ug/ml) 1% L-glutamine (200mM, 100x) 1% non-essential amino acid (100x) 1% nucleosides (100x) 1% b-mercaptoethanol (0.1M) 0.2
Wholemount in situ hybridisation
). Incubate at 37°C for 15 minutes. Pack a Sephadex G50 column equilibrated with 0.1% SDS, 50mM Tris.Cl (pH 7.5), 0.5mM EDTA (TES) by loading a 1ml syringe barrel (plugged with glass or plastic wool) and spinning @ 1500rpm 90sec. Dilute probe
solution (see recipe for the alkaline lysis solution; omit Triton X‐100 and adjust pH to 7) Neutral electrophoresis buffer: 300 mM sodium acetate/100 mM Tris⋅Cl, pH 8.5 (see appendix 2A
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