Matrices listed below were spiked with certain level of S1PR3 and the recovery rates were calculated by comparing the measured value to the expected amount of S1PR3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-104
95
EDTA plasma(n=5)
96-101
98
heparin plasma(n=5)
95-103
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of S1PR3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.