Matrices listed below were spiked with certain level of LPL and the recovery rates were calculated by comparing the measured value to the expected amount of LPL in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-104
96
EDTA plasma(n=5)
85-103
93
heparin plasma(n=5)
89-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LPL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.