Matrices listed below were spiked with certain level of IMPA2 and the recovery rates were calculated by comparing the measured value to the expected amount of IMPA2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-100
96
EDTA plasma(n=5)
85-102
93
heparin plasma(n=5)
87-105
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IMPA2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.