Matrices listed below were spiked with certain level of ITGA2B and the recovery rates were calculated by comparing the measured value to the expected amount of ITGA2B in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-97
91
EDTA plasma(n=5)
87-103
95
heparin plasma(n=5)
86-104
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ITGA2B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.