Matrices listed below were spiked with certain level of ALDH1A1 and the recovery rates were calculated by comparing the measured value to the expected amount of ALDH1A1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-100
90
EDTA plasma(n=5)
87-104
93
heparin plasma(n=5)
85-103
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ALDH1A1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.