Matrices listed below were spiked with certain level of MUC5B and the recovery rates were calculated by comparing the measured value to the expected amount of MUC5B in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-103
97
EDTA plasma(n=5)
90-102
96
heparin plasma(n=5)
89-104
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MUC5B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.