Matrices listed below were spiked with certain level of KLF10 and the recovery rates were calculated by comparing the measured value to the expected amount of KLF10 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-100
96
EDTA plasma(n=5)
87-103
96
heparin plasma(n=5)
88-101
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KLF10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.