Matrices listed below were spiked with certain level of KLB and the recovery rates were calculated by comparing the measured value to the expected amount of KLB in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-102
95
EDTA plasma(n=5)
87-100
93
heparin plasma(n=5)
94-103
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KLB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.