Matrices listed below were spiked with certain level of IL20RA and the recovery rates were calculated by comparing the measured value to the expected amount of IL20RA in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
93-103
98
EDTA plasma(n=5)
89-104
96
heparin plasma(n=5)
87-104
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL20RA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.