Matrices listed below were spiked with certain level of SAA4 and the recovery rates were calculated by comparing the measured value to the expected amount of SAA4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-101
93
EDTA plasma(n=5)
94-101
98
heparin plasma(n=5)
89-99
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SAA4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.