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文献和实验Analysis of Cell Cycle by Flow Cytometry
Described are four widely used procedures to analyze the cell cycle by flow cytometry. The first two are based on univariate analysis of cellular DNA content following cell staining with either propidium iodide (PI) or 4′,6′-diamidino
PROPIDIUM IODIDE: The most commonly used dye for DNA content/cell cycle analysis is PROPIDIUM IODIDE (PI). It can be used to stain whole cells or isolated nuclei. The PI intercalates into the major groove of double-stranded DNA and produces
Cell Cycle Analysis of Asynchronous Populations
Measurement of a DNA histogram can be achieved by fixing or permeabilizing cells and staining them with a DNA-binding dye, such as propidium iodide (PI). The histogram will yield the percentage of cells in the G1, S, and G2/M phases
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