- ￥1599
- merck millipore
- AB142
- 2025年07月07日
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文献和实验实验试剂 1. 2-mercaptoethanol 2. Absolute (96%-100%) ethanol 3. Liquid nitrogen for freezing/disrupting samples 实验设备 1. Microcentrifuge capable of 10,000 x g 2. Nuclease-free microfuge
Modified protocol for Isolating RNA from Grape Leaves
for 5 minutes. Remove the supernatant and dissolve RNA by 300 ul DEPC Water. 7. Add equal volume of the RB Buffer followed by 325 ul absolute ethanol (96-100%). Mix the sample thoroughly by vortexing. 8. Load 600 ul of the sample into the HiBind RNA
each wash. Add Rb IgG (4 ug) or anti-XKCM1 Gly (4 ug) and bring volume to 100 ul total. Bind antibody to beads at 4 deg.C for 1 hr 15' on rotator. Make sure beads are rolling around. Pellet in ufuge in coldroom and wash 1X TBST, 3X CSFXB
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