Matrices listed below were spiked with certain level of HDAC8 and the recovery rates were calculated by comparing the measured value to the expected amount of HDAC8 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-105
95
EDTA plasma(n=5)
86-102
93
heparin plasma(n=5)
89-103
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HDAC8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.