Matrices listed below were spiked with certain level of AFP-L3 and the recovery rates were calculated by comparing the measured value to the expected amount of AFP-L3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
95
EDTA plasma(n=5)
92-100
96
heparin plasma(n=5)
85-100
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AFP-L3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.