Matrices listed below were spiked with certain level of EIF2AK3 and the recovery rates were calculated by comparing the measured value to the expected amount of EIF2AK3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-102
95
EDTA plasma(n=5)
90-105
95
heparin plasma(n=5)
90-101
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EIF2AK3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.