Human Phospho-KIF11 / EG5 (Thr
926) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1404- $740
- LSBio
- To be determined
- LS-F1404
- 美国
- 2025年07月16日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Human
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
To be determined
- 供应商：
康朗生物
- 规格：
96T
Human Phospho-KIF11 / EG5 (Thr926) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1404
Print This Page
Technical Help
Review This Product
SpecificationsPublicationsReviewsImages
Product Description
LS-F1404 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human KIF11 / EG5 in samples of Adherent Cell Cultures. It is based upon a Cell-Based Phosphorylation assay principle.Specifications
TypeCell-Based Phoshorylation ELISA (enzyme-linked immunosorbent assay) kit
Target
KIF11 / EG5
Reactivity
Human
Specificity
KIF11/Eg5 (Phospho-Thr926)
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Qualitative
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Cell Culture Microplate
- Blocking Buffer
- Detection Antibodies
- HRP-Conjugate
- Substrate
- Stop Solution
- Crystal Violet Solution
- SDS Solution
- Adhesive Plate Sealers
Protocol
- Seed cells into wells and incubate overnight at 37°C, 5% CO2.
- Apply desired treatment conditions.
- Add 100 µl of Fixing Solution and incubate 20 minutes at room temperature.
- Add 100 µl of Quenching Buffer and incubate 20 minutes at room temperature.
- Add 200 µl of Blocking Buffer and incubate for 1 hour at room temperature.
- Add 50 µl of 1x primary antibodies and incubate overnight at 4°C. Add 50 µl of HRP-conjugated secondary antibodies and incubate for 1.5 hours at room temperature.
- Add 50 µl of Ready-to-Use Substrate and incubate for 30 minutes at room temperature.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About KIF11 / EG5
P52732 NM_004523 NP_004514.2
Popular KIF11 / EG5 Elisa Kits
Human KIF11 / EG5 ELISA Kit (Cell-Based ELISA) - LS-F2108
Reactivity: Human
Range: Positive/Negative
More KIF11 / EG5 Products
Publications (0)
Submit a Publication
Customer Reviews (0)
Submit a Review
Top of Page
Images
Cell-Based Phosphorylation ELISA Platform Overview
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 and Total-p38 antibodies (C). Cell-Based Elisa将小鼠巨噬细胞4/4细胞种在96孔板内，每个孔种5 x 104 cells/cm2细胞。细胞用不同浓度的anisomycin刺激，我们来监测P38 MAPK和JNK两种蛋白的变化。按照试剂盒的实验步骤，分别固定，打孔，猝灭，封闭，然后一抗孵育，二抗孵育，最后显色，用常规的酶标仪读取数值。最后数据再和试剂盒提供的结晶紫核染料结果做纠正，最后得出以上数据。Quantitative
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation
Determining In Vivo Phosphorylation Sites Using Mass Spectrometry
Basic Protocol 2: Global Phosphorylation Analysis (Ser/Thr/Tyr) Alternate Protocol 2: SDS‐PAGE Protein Fractionation of Whole‐Cell Extracts Basic Protocol 3: Phosphotyrosine (pTyr) Site Identification
技术资料暂无技术资料 索取技术资料
