Matrices listed below were spiked with certain level of BMP1 and the recovery rates were calculated by comparing the measured value to the expected amount of BMP1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-99
95
EDTA plasma(n=5)
92-105
100
heparin plasma(n=5)
95-105
100
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BMP1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.