LS-F10006 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Insulin C-Peptide in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of Insulin C-Peptide as low as 0.25 nanograms per millilter.
This assay has high sensitivity and excellent specificity for detection of human C-Peptide. No significant cross-reactivity or interference between human C-Peptide and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human C-Peptide and all the analogs, therefore, cross reaction may still exist.
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Sample Size
50 - 100 µl
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.7 - 10 ng/ml
Sensitivity
0.25 ng/ml
Precision
Intra-assay: CV%<15% Inter-assay: CV%<15%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
User Manual
96-well Microplate
Detection Reagents
Wash Buffer
Substrate
Stop Solution
Adhesive Plate Sealers
Background
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C-Peptide has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for C-Peptide. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C-Peptide bound in the initial step. The color development is stopped and the intensity of the color is measured.
Restrictions