Matrices listed below were spiked with certain level of GGT1 and the recovery rates were calculated by comparing the measured value to the expected amount of GGT1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-105
96
EDTA plasma(n=5)
86-105
97
heparin plasma(n=5)
90-105
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GGT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.