- $615
- LSBio
- To be determined
- LS-F2089
- 美国
- 2025年06月27日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Mouse, Human
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
To be determined
- 供应商：
康朗生物
- 规格：
96T
Mouse/Human ITCH / AIP4 ELISA Kit (Cell-Based ELISA)
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Product Description
LS-F2089 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human ITCH / AIP4 in samples of Adherent Cell Cultures. It is based upon a Cell-Based assay principle.Specifications
TypeCell-Based ELISA (enzyme-linked immunosorbent assay) kit
Target
ITCH / AIP4
Reactivity
Mouse, Human
Specificity
ITCH
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Qualitative
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Cell Culture Microplate
- Blocking Buffer
- Detection Antibodies
- HRP-Conjugate
- Substrate
- Stop Solution
- Crystal Violet Solution
- SDS Solution
- Adhesive Plate Sealers
Protocol
- Seed cells into wells and incubate overnight at 37°C, 5% CO2.
- Apply desired treatment conditions.
- Add 100 µl of Fixing Solution and incubate 20 minutes at room temperature.
- Add 100 µl of Quenching Buffer and incubate 20 minutes at room temperature.
- Add 200 µl of Blocking Buffer and incubate for 1 hour at room temperature.
- Add 50 µl of 1x primary antibodies and incubate overnight at 4°C. Add 50 µl of HRP-conjugated secondary antibodies and incubate for 1.5 hours at room temperature.
- Add 50 µl of Ready-to-Use Substrate and incubate for 30 minutes at room temperature.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About ITCH / AIP4
Q96J02 NM_031483 NP_113671.3
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Cell-Based ELISA Platform Overview
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文献和实验A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
. to 1 liter. 4. 30% H2O2 5. OPD Stock, 4.0% 4 g OPD in 100 ml di-H2O. Aliquot and store at -20oC. Protect from light. 6. 4.5N H2SO4 12.0 ml Concentrated Sulfuric Acid 88.0 ml di-H2O 7. Hanks Balanced Salt Solution (HBSS) B. Procedure 1. Wash ELISA
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