Matrices listed below were spiked with certain level of PYY3 and the recovery rates were calculated by comparing the measured value to the expected amount of PYY3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-101
94
EDTA plasma(n=5)
89-102
94
heparin plasma(n=5)
88-105
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PYY3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.