Matrices listed below were spiked with certain level of CNN1 and the recovery rates were calculated by comparing the measured value to the expected amount of CNN1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
96-100
98
EDTA plasma(n=5)
86-104
95
heparin plasma(n=5)
89-97
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CNN1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.