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欣博盛
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大量
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1 mg
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文献和实验Yeast mini whole cell extract using glass beads for western analysis
----------------------- Extraction Buffer : 200 mM Tris pH 8.0 150 mM Ammonium sulfate 10% glycerol 1 mM EDTA Add fresh on day of extract preparation : 2 mM DTT PMSF Benzamidine Leupeptin Pepstatin Chymostatin Protease inhibitors 0.1 M PMSF (100x) 16 mg/ml Ethanol
Yeast mini whole cell extract using glass beads for western analysis
preparation : 2 mM DTT PMSF Benzamidine Leupeptin Pepstatin Chymostatin Protease inhibitors 0.1 M PMSF (100x) 16 mg/ml Ethanol Store at -20 degrees 0.2M DTT (100x) 32 mg/ml H2O Store frozen at -20 degrees Benzamidine
benzamidine) 0.6uM leupeptin (= 0.3μg/ml or 1/500 dilution of 0.15 mg/ml leupeptin in ethanol) 2uM pepstatin A (= 1.4μg/ml or 1/200 dilution of 0.28 mg/ml pepstatin A in methanol) 3.3uM chymostatin (= 2μg/ml or 1/2,500 dilution of 5mg/ml chymostatin in DMSO
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